|
KMID : 0604520190450010049
|
|
Journal of the Society of Cosmetic Scientists of Korea
2019 Volume.45 No. 1 p.49 ~ p.56
|
|
|
A Study on the Whitening Effects of Pueraria thomsonii Extract and its Three Tectorigenin Derivatives
|
|
Ahn Young-Je
Chang Yun-Hee
Lee So-Young
Jin Moo-Hyun
|
|
|
Abstract
|
|
|
|
Pueraria thomsonii Benth. as a medicinal ingredient, has been traditionally used in Chinese medicine to treat fever, acute dysentery, diarrhea, diabetes, and cardiovascular disease. The effects of P. thomsonii flower on skin have not been reported yet. In this study, the whitening effect of P. thomsonii flower was verified using B16F1 melanoma cells and HS68 fibroblasts. P. thomsonii flower extract reduced melanin contents of B16F1 cells in a dose-dependent manner. To identify its active components, we analyzed P. thomsonii flower extract using high performance liquid chromatography (HPLC). As a result, we identified three major isoflavones of tectorigenin, tectoridin, and tectorigenin 7-O-xylosylglucoside. At a non-cytotoxic concentration, the three components also reduced melanin contents of B16F1 cells in a dose-dependent manner. The depigmentation effects were attributed to the reduced gene expression of tyrosinase and microphthalmia-associated transcription factor (MITF). In order to elucidate another depigmentation mechanism, their effects on DKK-1, a fibroblast-derived depigmentation factor, was determined in HS68 cells. As a result, P. thomsonii flower extracts, tectoridin and tectorigenin 7-O-xylosylglucoside, reduced DKK-1 gene expression, while tectorigenin increased DKK-1 gene expression in a dose-dependent manner. These results suggest that tectorigenin can be used as an effective whitening agent that inhibit melanin synthesis in melanocytes and promote the secretion of depigmentation factor from fibroblasts.
|
|
|
KEYWORD
|
|
|
Pueraria thomsonii Benth, tectorigenin, tectoridin, tectorigenin 7-O-xylosylglucoside, tyrosinase
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|